Urine as a high-quality source of host genomic DNA from wild populations.


Ozga, AT; Webster, TH; Gilby, IC; Wilson, MA; Nockerts, RS; Wilson, ML; Pusey, AE; Li, Y; Hahn, BH; Stone, AC


The ability to generate genomic data from wild animal populations has the potential to give unprecedented insight into the population history and dynamics of species in their natural habitats. However, for many species, it is impossible legally, ethically or logistically to obtain tissue samples of quality sufficient for genomic analyses. In this study we evaluate the success of multiple sources of genetic material (faeces, urine, dentin and dental calculus) and several capture methods (shotgun, whole-genome, exome) in generating genome-scale data in wild eastern chimpanzees (Pan troglodytes schweinfurthii) from Gombe National Park, Tanzania. We found that urine harbours significantly more host DNA than other sources, leading to broader and deeper coverage across the genome. Urine also exhibited a lower rate of allelic dropout. We found exome sequencing to be far more successful than both shotgun sequencing and whole-genome capture at generating usable data from low-quality samples such as faeces and dental calculus. These results highlight urine as a promising and untapped source of DNA that can be noninvasively collected from wild populations of many species.


Ozga, Andrew T., Timothy H. Webster, Ian C. Gilby, Melissa A. Wilson, Rebecca S. Nockerts, Michael L. Wilson, Anne E. Pusey, Yingying Li, Beatrice H. Hahn, and Anne C. Stone. “Urine as a high-quality source of host genomic DNA from wild populations.” Molecular Ecology Resources 21, no. 1 (January 2021): 170–82. https://doi.org/10.1111/1755-0998.13260.

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